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Image Search Results
Journal: iScience
Article Title: Casein kinase 1α is required to maintain murine hypothalamic pro-opiomelanocortin expression
doi: 10.1016/j.isci.2023.106670
Figure Lengend Snippet: Notch signaling pathway inhibition restores the phenotype of PKO mice (A and B) Active β-catenin protein expression in control and PKO hypothalami at E15.5 (A) and P60 (B). (C and D) Quantification of active β-catenin protein in control and PKO hypothalami at E15.5 (C) and P60 (D) (n = 3, ns p > 0.05). Data are means ± SEM. (E and F) NICD and α-tubulin protein expression in control and PKO hypothalami at E15.5 (E) and P60 (F). (G and H) Quantification of NICD protein expression in control and PKO hypothalami at E15.5 (G) and P60 (H) (n = 3, ∗p < 0.05, ∗∗p < 0.01). Data are means ± SEM. (I) NICD and POMC protein expression in hypothalami of control and PKO mice injected with NaCl or 10 mg/kg DAPT at E15.5. (J and K) Quantification of NICD (J) and POMC (K) protein in control and PKO hypothalami at E15.5 (n = 3, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). Data are means ± SEM. (L) POMC immunofluorescence staining in E15.5 control and PKO hypothalami injected with 10 mg/kg DAPT. Scale bars, 50 μm. (M) Numbers of POMC + neurons in E15.5 control and PKO hypothalami after the injection of NaCl or 10 mg/kg DAPT (n = 4, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). Data are means ± SEM. Control = Csnk1a1 fl/+ ;POMC cre . PKO = Csnk1a1 fl/fl ;POMC cre .
Article Snippet:
Techniques: Inhibition, Expressing, Control, Injection, Immunofluorescence, Staining
Journal: iScience
Article Title: Casein kinase 1α is required to maintain murine hypothalamic pro-opiomelanocortin expression
doi: 10.1016/j.isci.2023.106670
Figure Lengend Snippet:
Article Snippet:
Techniques: Biomarker Discovery, Recombinant
Journal: Journal of Cellular and Molecular Medicine
Article Title: BMSC s and miR‐124a ameliorated diabetic nephropathy via inhibiting notch signalling pathway
doi: 10.1111/jcmm.13747
Figure Lengend Snippet: The primer sequences in qRT‐PCR assay
Article Snippet: Next, immunoblotting was carried out using antibody to Pdx‐1 (rabbit anti‐ Pdx‐1 antibody, 1:250; Abcam), Pax‐6 (rabbit anti‐ Pax‐6 antibody, 1:250; Abcam), insulin‐1 (rabbit anti‐ insulin‐1 antibody, 1:250; Abcam), Ngn3 (rabbit anti‐Ngn3 antibody, 1:250; Abcam), GK (rabbit anti‐GK antibody, 1:250; Abcam), nephrin (rabbit anti‐nephrin antibody, 1:400; Abcam), podocin (rabbit anti‐podocin antibody, 1:250; Abcam), CD2AP (rabbit anti‐CD2AP antibody, 1:250; Abcam), caspase‐3 (rabbit anti‐caspase‐3 antibody, 1:400; Beyotime, Shanghai), Bax (rabbit anti‐Bax antibody, 1:400; Beyotime, Shanghai), Bcl‐2 (rabbit anti‐Bcl‐2 antibody, 1:400; Beyotime, Shanghai), Notch1 (rabbit anti‐Notch1 antibody, 1:400; Beyotime, Shanghai),
Techniques:
Journal: Journal of Cellular and Molecular Medicine
Article Title: BMSC s and miR‐124a ameliorated diabetic nephropathy via inhibiting notch signalling pathway
doi: 10.1111/jcmm.13747
Figure Lengend Snippet: BMSC s in combination with miR‐124a regulated Notch1, NICD , Hes1 and Delta expressions. Podocytes cells were treated with PBS (Con) and 40 mmol/L HG ( HG ) for 24 h and were then co‐cultured with BMSC s, which were transfected with mock (M/ HG ), miR‐124a mimics (m/ HG ) and miR‐124a inhibitors (i/ HG ) for 48 h, respectively. A, The mRNA expression levels of Notch1, NICD , Hes1 and Delta were measured by qRT ‐ PCR assay, respectively (** P < .01 vs Con; ^ P < .05 or ^^ P < .01 vs HG ; & P < .05 or && P < .01 m/ HG vs M/ HG ; ## P < .01 i/ HG vs M/ HG ). B, The protein expression levels of Notch1, NICD , Hes1 and Delta were detected by Western blot assay
Article Snippet: Next, immunoblotting was carried out using antibody to Pdx‐1 (rabbit anti‐ Pdx‐1 antibody, 1:250; Abcam), Pax‐6 (rabbit anti‐ Pax‐6 antibody, 1:250; Abcam), insulin‐1 (rabbit anti‐ insulin‐1 antibody, 1:250; Abcam), Ngn3 (rabbit anti‐Ngn3 antibody, 1:250; Abcam), GK (rabbit anti‐GK antibody, 1:250; Abcam), nephrin (rabbit anti‐nephrin antibody, 1:400; Abcam), podocin (rabbit anti‐podocin antibody, 1:250; Abcam), CD2AP (rabbit anti‐CD2AP antibody, 1:250; Abcam), caspase‐3 (rabbit anti‐caspase‐3 antibody, 1:400; Beyotime, Shanghai), Bax (rabbit anti‐Bax antibody, 1:400; Beyotime, Shanghai), Bcl‐2 (rabbit anti‐Bcl‐2 antibody, 1:400; Beyotime, Shanghai), Notch1 (rabbit anti‐Notch1 antibody, 1:400; Beyotime, Shanghai),
Techniques: Cell Culture, Transfection, Expressing, Quantitative RT-PCR, Western Blot